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Chemical Triphosphorylation of Oligonucleotides
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酵素アーキテクチャ:カチオン-フォスフォディアニオンペアから移行状態の安定化の最適化

Archie C Reyes1, Astrid P Koudelka1, Tina L Amyes1

  • 1Department of Chemistry, University at Buffalo, SUNY, Buffalo, New York 14260-3000, United States.

Journal of the American Chemical Society
|April 18, 2015
PubMed
まとめ

l-グリセロール3リン酸脱水素酵素 (GPDH) は,移行状態を安定させるために基板結合エネルギーを使用します. 変異は,GPDHが静電相互作用を通じて移行状態を積極的に安定させ,触媒効率を高める方法を明らかにします.

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