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バクテリアの分裂. 機械的な亀裂の伝播は,Staphylococcus aureusの娘細胞分離をミリ秒で推進する
Xiaoxue Zhou1, David K Halladin2, Enrique R Rojas3
1Department of Chemistry, Stanford University, Stanford, CA 94305, USA. Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Howard Hughes Medical Institute (HHMI), Stanford University School of Medicine, Stanford, CA 94305, USA.
Science (New York, N.Y.)
|May 2, 2015
まとめ
Staphylococcus aureusは,細胞壁のストレスのために"ポップアップ"することによって,娘細胞に急速に分離します. この超高速なプロセスは,細胞をつなぐ周辺のリングで機械的な亀裂の拡散を伴う.
科学分野:
- 微生物学 微生物学とは
- 細胞生物学 細胞生物学
- バイオフィジックス 生物物理学
背景:
- Staphylococcus aureusは,細胞運動を行い,外周環で接続された2つの子細胞を形成します.
- この細胞分離のメカニズムとダイナミクスは完全に理解されていません.
研究 の 目的:
- Staphylococcus aureusの娘細胞の急速な分離を駆動する物理的メカニズムを調査する.
- 細胞壁のストレスと,細胞運動における機械的障害の役割を分析する.
主な方法:
- 高速画像を用いた細胞分離ダイナミクスの観察.
- 分離中の細胞体積変化の分析.
- エラストスタティックモデルを細胞壁に適用する.
- 外周環の故障点を顕微鏡で検査する.
主要な成果:
- 娘細胞分離と呼ばれる娘細胞分離です.
- ポップピング・ポップピング
- , は数ミリ秒で,音量の大きな変化なしに発生します.
- 分離の確率は細胞壁のストレスと相関し,非対称な分裂とヒンジ形成につながります.
- 弾性静止モデリングは,外周環における高回転張力を示しています.
- 初期の機械的な故障点は,小さな穿孔として観察され,外周環で特定されました.
結論:
- Staphylococcus aureusの超高速な娘細胞分離は,外周環のストレス蓄積によって引き起こされる.
- このプロセスは,機械的なクラック伝播の特徴を示しています.
- 細胞壁のメカニズムは,細菌の細胞運動と細胞分裂において重要な役割を果たします.
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