Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Microtubule Instability02:17

Microtubule Instability

6.5K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
6.5K
Microtubule Instability02:17

Microtubule Instability

6.3K
6.3K
Microtubules01:18

Microtubules

11.5K
Microtubules are the thickest cytoskeletal filaments with a diameter of 25 nm. In prokaryotic organisms, microtubules are commonly found in locomotory appendages like cilia and flagella. In eukaryotic cells, microtubules form specialized extensions for moving fluid over the surface, like those found in cells lining the intestine.
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer....
11.5K
Microtubules01:35

Microtubules

103.5K
There are three types of cytoskeletal structures in eukaryotic cells—microfilaments, intermediate filaments, and microtubules. With a diameter of about 25 nm, microtubules are the thickest of these fibers. Microtubules carry out a variety of functions that include cell structure and support, transport of organelles, cell motility (movement), and the separation of chromosomes during cell division.
103.5K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

2.9K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.9K
Microtubule Formation01:23

Microtubule Formation

8.2K
Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation...
8.2K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Cryo-EM of the eukaryotic purine transporter UapA demonstrates intramolecular and lipid regulation of transport.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A milestone in C4 carbon concentration mechanism evolution: structural remodeling of NADP-malic enzyme in Poaceae.

Molecular biology and evolution·2026
Same author

Ideal efficacy photoswitching for chromocontrol of TRPC4/5 channel functions in live tissues.

Nature chemical biology·2026
Same author

Monte Carlo simulations of transverse relaxation for characterization of physicochemical properties of superparamagnetic iron oxide nanoparticles.

Magnetic resonance imaging·2025
Same author

Structural basis of apoptosis induction by the mitochondrial voltage-dependent anion channel.

Nature communications·2025
Same author

Spatial N-glycan rearrangement on α<sub>5</sub>β<sub>1</sub> integrin nucleates galectin-3 oligomers to determine endocytic fate.

Nature communications·2025
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
関連記事をすべて見る

関連する実験動画

Updated: Apr 12, 2026

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles
07:54

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

Published on: November 5, 2020

5.8K

チューブリンコードの解読

Stefan Raunser1, Christos Gatsogiannis1

  • 1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, 44227 Dortmund, Germany.

Cell
|May 23, 2015
PubMed
まとめ
この要約は機械生成です。

研究者らは,微小管の改変に不可欠なチューブリンチロシンリガゼのような (TTLL) 酵素の構造を明らかにした. これは,これらの酵素が微小管と相互作用して細胞機能を調節する方法に関する新しい洞察を提供します.

さらに関連する動画

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
08:02

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy

Published on: March 3, 2023

2.0K
Author Spotlight: Purifying High-Quality Tubulin to Study Protein Dynamics and Therapeutic Applications
06:30

Author Spotlight: Purifying High-Quality Tubulin to Study Protein Dynamics and Therapeutic Applications

Published on: October 11, 2024

2.3K

関連する実験動画

Last Updated: Apr 12, 2026

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles
07:54

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

Published on: November 5, 2020

5.8K
Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
08:02

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy

Published on: March 3, 2023

2.0K
Author Spotlight: Purifying High-Quality Tubulin to Study Protein Dynamics and Therapeutic Applications
06:30

Author Spotlight: Purifying High-Quality Tubulin to Study Protein Dynamics and Therapeutic Applications

Published on: October 11, 2024

2.3K

科学分野:

  • バイオケミストリー バイオケミストリー
  • 細胞生物学 細胞生物学
  • 構造生物学 構造生物学とは

背景:

  • 微小管は,様々な細胞プロセスに関与する重要な細胞骨格の構成要素です.
  • グルタミレーションなどの翻訳後の改変は,微小管の機能を調節する.
  • チューブリンチロシンリガゼ型 (TTLL) 酵素ファミリーは,マイクロチューブルのグルタミレーションを触媒化する.

研究 の 目的:

  • TTLL酵素活性の構造的基礎を決定する.
  • TTLL酵素が微小管と相互作用するメカニズムを解明する.
  • グルタミレーションが微小管を特定タンパク質の相互作用にどのようにマークするかを理解するために.

主な方法:

  • TTLLタンパク質の構造を得るために,X線結晶学を用いた.
  • TTLL-マイクロチューブル複合体を視覚化するために,冷凍電子顕微鏡 (cryo-EM) が使用されました.
  • 酵素活性と基質結合を評価するために生化学的測定を行いました.

主要な成果:

  • TTLL酵素の最初の原子構造が決定されました.
  • マイクロチューブルと複合したTTLL酵素の構造は,重要な相互作用インターフェースを明らかにした.
  • この研究では,TTLL媒介のグルタミレーションの触媒メカニズムが明らかにされました.

結論:

  • 構造的および機械的洞察は,微小管の調節に関する理解を深める.
  • この研究は,TTLLおよび健康と病気におけるその役割に関するさらなる研究のための基盤を提供します.
  • この発見は,微小管のダイナミクスを標的とした新しい治療戦略の開発の道を開く.