単細胞トランスクリプトームの分析により,休眠している神経幹細胞を活性化するための信号が明らかになりました
Yuping Luo1, Volkan Coskun2, Aibing Liang3
1Stem Cell Translational Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China; College of Life Sciences, Nanchang University, Nanchang 330031, China.
Cell
|May 23, 2015
まとめ
大人の脳内の休眠状態の末端神経幹細胞 (NSC) が特定されました. 血管内皮成長因子 (VEGF) と基礎線維細胞成長因子 (bFGF) は,これらのNSCを活性化し,神経系統の微分化と移動を促進し,新しい治療の機会を提供します.
科学分野:
- 神経科学は神経科学である.
- 幹細胞生物学 幹細胞生物学
- 分子生物学は分子生物学である.
背景:
- 組織特異性幹細胞の特徴づけは,その希少性と複雑なマイクロ環境のために困難です.
- 成人マウスの前頭脳神経原性ゾーンのエペンジマル細胞は,神経幹細胞 (NSC) の潜在的な源である.
研究 の 目的:
- 成人マウスの前脳における静止状態のエペンジマル細胞 (CD133(+) /GFAP(-)) を分子的に特徴付ける.
- エペンジマのNSCの活性化と分化の可能性を調査する.
主な方法:
- 単細胞トランスクリプトーム解析.
- ウェイトゲン共同発現ネットワーク分析 (WGCNA).
- 血管内皮成長因子 (VEGF) と基礎線維芽細胞成長因子 (bFGF) のインビヴォ投与.
主要な成果:
- 静止状態のエペンジマルCD133 ((+) /GFAP ((-) 細胞は,免疫反応性および血管新生因子受容体遺伝子に富んだユニークな遺伝子ネットワークを有しています.
- VEGFの投与により,側心室と第4心室の全域でエペンジマのNSCが活性化しました.
- VEGFとbFGFの併用により,神経系統の微分化と移行が誘発された.
結論:
- 休眠状態のエペンジマルNSCは,中枢神経系 (CNS) の心室表面全体に存在します.
- 免疫および血管新生信号,特にVEGFは,これらの眠っているNSCを活性化させることができます,特に怪我後の場合.
- この発見は,脳の修復と再生戦略の新たな可能性を開きます.
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