単一のキナーゼは,分泌されるフォスフォプロテオームの過半数を生成する
Vincent S Tagliabracci1, Sandra E Wiley1, Xiao Guo2
1Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA.
Cell
|June 20, 2015
まとめ
Fam20Cは,ほとんどの細胞外タンパク質のリン酸化を司る主要なキナーゼとして特定されています. この発見は,様々な生物学的プロセスや疾患における分泌されるタンパク質の役割を理解するための新しい道を開きます.
科学分野:
- バイオケミストリー バイオケミストリー
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- 細胞外フォスフォタンパク質は1世紀以上前から知られていましたが,その研究は,特定されたキナーゼの欠如によって制限されていました.
- これらのキナーゼを特定することは,分泌されるタンパク質の機能と調節を理解するために重要である.
研究 の 目的:
- 細胞外タンパク質のリン酸化を司る主要なキナーゼを特定する.
- このキナーゼの基板と機能を特徴付けるために.
主な方法:
- CRISPR/Cas9によるゲノム編集
- 質量スペクトロメトリによる質量スペクトロメトリです.
- バイオケミカルアッセイ
主要な成果:
- Fam20Cは,細胞外フォスフォプロテオームの大部分に責任を負うキナーゼとして特定されました.
- 100以上の分泌されたフォスフォタンパク質がFam20C基板であると確認されました.
- Fam20Cは,これまで知られていたより広範な基質特異性を実証し,バイオミネラライゼーションを超えた役割を果たしました.
結論:
- Fam20Cは,主要な分泌経路タンパク質キナーゼとして確立されています.
- Fam20C基板は,脂質ホメオスタシス,傷の治癒,細胞の移動,粘着に関与しています.
- この研究は,ヒトの健康と疾患における分泌されたタンパク質のリン酸化を調査するための基礎を提供します.
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