産後神経幹細胞の胚性起源について
Luis C Fuentealba1, Santiago B Rompani2, Jose I Parraguez1
1Department of Neurological Surgery and the Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA.
Cell
|June 20, 2015
まとめ
ネズミの神経幹細胞 (B1細胞) は,胚の発達初期に指定され,嗅覚球の神経細胞の生成に影響を与えます. これらの細胞は,産後再活性化まで休眠状態であり,重要な系統関係を明らかにします.
科学分野:
- 神経科学は神経科学である.
- 発達生物学 発達生物学について
- 幹細胞生物学 幹細胞生物学
背景:
- 側心室にある成人神経幹/祖先 (B1) 細胞は,嗅覚球の神経細胞を生成する.
- B1細胞の位置が作られる嗅覚球の神経細胞の種類を決定する.
研究 の 目的:
- ネズミのB1細胞の発達の起源と系統関係を調査する.
- B1細胞の特異化と,胚の祖先とのつながりのタイミングを決定する.
主な方法:
- 系統追跡のために10万以上の遺伝子タグを備えたレトロウイルスライブラリを利用した.
- 胚の発達 (E11.5-E15.5) と産後段階におけるB1細胞前駆体を分析した.
主要な成果:
- 大半のB1細胞前駆体は,胚の13.5-15.5日間で生成され,産後再活性化まで静止状態にとどまります.
- B1細胞は,胚の皮質細胞,線状細胞,隔膜細胞と共通の祖先を共有していますが,このリンクはE15.5.5.の前に失われます.
- B1細胞の地域特異は,前頭脳のニューロン生産に関連したE11.5によって明らかである.
結論:
- 産後神経幹細胞の初期の胚性地域特異性を実証しています.
- B1細胞と胚の祖先細胞の間の一時的な系統関係を明らかにする.
- 神経幹細胞の運命決定における発達タイミングの重要性を強調する.
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