精密に定義されたタンパク質/ペプチド-ポリマー結合体による水性Cu-LRP:合成と制御された自己組み立て
Qiang Zhang1, Muxiu Li1, Chongyu Zhu1
1Department of Chemistry, University of Warwick, CV4 7AL, Coventry, United Kingdom.
Journal of the American Chemical Society
|July 8, 2015
まとめ
研究者は,水中の制御されたポリメリゼーションを使用して,新しいタンパク質-ポリマー結合体を作成しました. この方法により,インスリンポリマー球のような材料の調整可能な自己組み立てが可能になり,様々な用途に使用できます.
科学分野:
- バイオマテリアル科学 バイオマテリアル科学
- ポリマー化学のポリマー化学について
- 超分子化学 超分子化学
背景:
- タンパク質/ペプチド-ポリマー結合体は,高度な材料にとって価値があります.
- 水性媒体での自己組み立て行動を制御することは,依然として課題です.
- リビングラジカルポリメリゼーションは,精密なポリマー合成を提供します.
研究 の 目的:
- 水中のタンパク質/ペプチド-ポリマー結合体を合成するための堅牢な方法を開発する.
- これらの結合物の自己組立特性を調査するために.
- タンパク質の種類とポリメリゼーション条件が結合物質形成に及ぼす影響を調査する.
主な方法:
- シングル電子移転生ラジカルポリメリゼーション (SET-LRP) が採用されました.
- タンパク質/ペプチドは,ポリメリゼーションのマクロイニシアターとして機能しました.
- ポリメリゼーション条件は,さまざまなタンパク質/ペプチド基板に最適化されました.
主要な成果:
- 精密に定義されたタンパク質/ペプチド-ポリマー結合体は,水で成功裏に合成されました.
- 制御された分子量と狭い分子量分布が達成されました.
- 合成されたインスリン-ポリマー結合体は,調節可能な自己組み立てで球状の構造を形成した.
結論:
- SET-LRPは,機能的なタンパク質-ポリマー結合体を生成するための強力な戦略です.
- 結合体の自己組み立て行動は,外部の刺激によって調節することができる.
- このアプローチにより,新しいタンパク質ベースのバイオマテリアルの設計が可能になります.
関連する概念動画
00:57Cationic Chain-Growth Polymerization: Mechanism
3.1K
The cationic polymerization mechanism consists of three steps: initiation, propagation, and termination. In the initiation step of the polymerization process, the π bond of a monomer gets protonated by the Lewis acid catalyst, which is formed from boron trifluoride and water. The protonation of the π bond generates a carbocation stabilized by the electron‐donating group. In the propagation step, the π bond of the second monomer acts as a nucleophile and attacks the...
3.1K
01:09Radical Chain-Growth Polymerization: Mechanism
3.8K
The radical chain-growth polymerization mechanism consists of three steps: initiation, propagation, and termination of polymerization. The polymerization initiates when a free radical generated from the radical initiator adds to the unsaturated bond in the monomer. The unpaired electron of the free radical and one π electron in the unsaturated bond creates a σ bond between the free radical and the monomer. As a result, the other π electron in the unsaturated bond converts this species into...
3.8K
01:10Radical Chain-Growth Polymerization: Overview
3.7K
Chain-growth or addition polymerization is successive addition reactions of monomers with a polymer chain. In radical chain-growth polymerization, the reaction proceeds via a free-radical intermediate. The free radical is formed from radical initiators, which spontaneously generate free radicals by homolytic fission. Organic peroxides (such as dibenzoyl peroxide, as shown in Figure 1) or azo compounds are popular radical initiators. A low concentration ratio of radical initiator to monomer is...
3.7K
01:04Anionic Chain-Growth Polymerization: Mechanism
2.6K
The mechanism for anionic chain-growth polymerization involves initiation, propagation, and termination steps. In the initiation step, a nucleophilic anion, such as butyl lithium, initiates the polymerization process by attacking the π bond of the vinylic monomer. As a result, a carbanion, stabilized by the electron‐withdrawing group, is generated. The resulting carbanion acts as a Michael donor in the propagation step and attacks the second vinylic monomer, which acts as a Michael...
2.6K
01:28ATP and Macromolecule Synthesis
7.4K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
7.4K
02:35Free-Radical Chain Reaction and Polymerization of Alkenes
10.3K
The conversion of alkenes to macromolecules called polymers is a reaction of high commercial importance. The structure of the polymer is defined by a repeating unit, while the terminal groups are considered insignificant. The average degree of polymerization represents the number of repeating units in the polymer molecule and is denoted by the subscript n.
10.3K