キネトコア局所化PP1-Sds22カップルの染色体分離から極性リラックスまで

Nelio T L Rodrigues¹, Sergey Lekomtsev¹, Silvana Jananji²

¹MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.

Nature

|July 14, 2015

まとめ

新しい細胞分裂経路は,PP1-Sds22フォスファタゼを使用して,細胞皮質を柔らかくし,染色体分離と有秩序な細胞分裂を可能にします. このメカニズムは,染色体の動きと皮質の偏極化を結びつける.

科学分野:

細胞生物学細胞生物学
分子生物学は分子生物学である.
遺伝学遺伝学とは

背景:

細胞分裂には,染色体分離と細胞運動の間の正確な調整が必要です.
Ect2は,アクトミオシンリングの組立と収縮をシグナルするためにマイクロチューブルをスパインドルするために採用します.

研究の目的:

細胞分裂を調節する新しいシグナル伝達経路を特定する.
染色体分離と細胞分裂を結びつけるメカニズムを調査する.

主な方法:

増殖中のドロソフィラとヒトの細胞の細胞分裂を研究した.
フォロー形成,センターソーム,マイクロチューブルから独立したシグナル伝達経路を調査した.

主要な成果:

PP1フォスファタゼとSds22を含む並列信号伝達経路を発見した.
キネトコア局所化されたPP1-Sds22は,エズリン/ラジシン/モエシンタンパク質をデフォスフォリレートし,極性細胞皮質を柔らかくする.
この経路は,アナフェーズ延長と有秩序な細胞分裂を促進します.

結論:

保存されたキネトコアベースのフォスファタゼ信号 (PP1-Sds22) と基板 (エズリン/ラジシン/モエシン) を特定しました.
この経路は,アナフェーズ染色体の動きと皮質の極化を結びつける.
皮質軟化による細胞分裂によるカップル染色体分離.

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