Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Eddy Currents01:25

Eddy Currents

2.7K
Since eddy currents occur only in conductors, magnets can separate metals from other materials. For example, in a recycling center, trash is dumped in batches down a ramp, beneath which lies a powerful magnet. Conductors in the trash are slowed by eddy currents, while nonmetals in the trash move on, separating from the metals. This works for all metals, not just ferromagnetic ones.
Other major applications of eddy currents appear in metal detectors and the braking systems of trains and roller...
2.7K
EDTA: Chemistry and Properties01:22

EDTA: Chemistry and Properties

4.1K
Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
4.1K
EDTA: Auxiliary Complexing Reagents01:26

EDTA: Auxiliary Complexing Reagents

1.6K
EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
1.6K
EDTA: Direct, Back-, and Displacement Titration01:30

EDTA: Direct, Back-, and Displacement Titration

6.5K
The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
6.5K
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

819
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
819
Electronic Distance Measuring Instruments01:30

Electronic Distance Measuring Instruments

771
Electronic Distance Measuring Instruments (EDMs) are essential tools in modern surveying, offering precise distance measurements by emitting electromagnetic signals and calculating the time required for these signals to travel to a target and return. Two primary types of signals are used in EDMs — light waves and microwaves — each suited to specific environmental and distance requirements. Light-wave-based EDMs utilize either infrared or laser light, providing high accuracy over...
771

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Mitochondrial L-2-hydroxyglutarate is a physiological signalling metabolite.

Nature·2026
Same author

Lactate transport inhibition therapeutically reprograms fibroblast metabolism in experimental pulmonary fibrosis.

Science translational medicine·2026
Same author

RNA-triggered cell killing with CRISPR-Cas12a2.

Nature·2026
Same author

Integrative Clinical-Molecular Modeling Identifies <i>LRRN4CL</i> as a Determinant of Structural and Functional Myocardial Improvement.

bioRxiv : the preprint server for biology·2026
Same author

Microbial metabolism of methotrexate produces a STAT3 signaling molecule that alleviates gut inflammation.

bioRxiv : the preprint server for biology·2026
Same author

Inhibition of FicD-mediated AMPylation and deAMPylation by isoprenoid diphosphates.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
関連記事をすべて見る

関連する実験動画

Updated: May 5, 2026

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
09:00

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Published on: April 16, 2018

9.3K

ETCに 落ちてるのか? ええ,あなたはDを知っている!

Jonathan G Van Vranken1, Jared Rutter1

  • 1Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT.

Cell
|August 2, 2015
PubMed
まとめ
この要約は機械生成です。

ミトコンドリアの電子輸送鎖は,急速に成長する細胞において,ATPの合成ではなく,アスパルテートを作るのに不可欠です. この発見は この重要な細胞経路の 核機能を再定義します

さらに関連する動画

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

2.1K
Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors
10:44

Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors

Published on: January 31, 2025

1.4K

関連する実験動画

Last Updated: May 5, 2026

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
09:00

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Published on: April 16, 2018

9.3K
Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

2.1K
Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors
10:44

Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors

Published on: January 31, 2025

1.4K

科学分野:

  • 生物化学
  • 細胞生物学
  • 代謝経路

背景:

  • ミトコンドリアの電子輸送鎖 (ETC) は,アデノシン三酸塩 (ATP) 生成における重要な役割として伝統的に認識されています.
  • 細胞増殖は 急速な成長を支えるために 複雑な代謝調整を必要とします

研究 の 目的:

  • 増殖する細胞におけるミトコンドリア電子輸送鎖 (ETC) の重要な機能を調査する.
  • ATP合成または他の機能が急速に分裂する細胞におけるETCの主要な要件であるかどうかを判断する.

主な方法:

  • 増殖の条件下で細胞代謝の分析
  • 細胞成長とメタボリットの生成に対するETC阻害の影響の評価

主要な成果:

  • 2つの独立した研究は,増殖細胞におけるETCの唯一の重要な機能がアスパルテート合成であることを示しています.
  • ETCによるATP生成は細胞成長の重要な要件ではないことが示唆されています.

結論:

  • 増殖細胞におけるミトコンドリア電子輸送鎖の主要な役割は,ATPの合成ではなく,アスパルテート生成である.
  • この発見は,細胞の代謝と成長におけるETCの根本的な重要性を再評価することを必要としています.