PAF1,RNAポリメラーゼIIによるプロモーター近接停止の分子調節剤
Fei Xavier Chen1, Ashley R Woodfin1, Alessandro Gardini2
1Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, 320 E. Superior Street, Chicago, IL 60611, USA.
Cell
|August 18, 2015
まとめ
Pol- II関連因子1 (PAF1) は,RNAポリメラーゼII (Pol II) の一時停止を制御することによって,遺伝子発現を調節する. PAF1のレベルが低下すると,Pol IIの放出が増加し,メタゾアにおける遺伝子転写が促進されます.
科学分野:
- 分子生物学
- 遺伝子規制
- メタゾアンの発達
背景:
- RNAポリメラーゼII (Pol II) のプロモーター近接停止は,遺伝子発現の重要な規制メカニズムである.
- この一時停止は メタゾウの環境と発達信号に 反応する遺伝子にとって 極めて重要です
研究 の 目的:
- プロモーター近接停止の調節におけるPol-II関連因子1 (PAF1) の役割を調査する.
- メタゾアンの遺伝子発現制御におけるPAF1の保存機能を解明する.
主な方法:
- メタゾーンモデルでPAF1レベルを低下させる効果を研究した.
- Pol II 放出,トランスクリプトレベル,および Pol II C 末端領域リン酸化 (Ser2P) の変化を分析した.
主要な成果:
- PAF1のレベルが低下すると,数千の遺伝子のゲノム体への停止したPol IIの放出が増加しました.
- PAF1の減少は,新生と成熟したトランスクリプトの上昇とSer2Pレベルの増加をもたらしました.
- 超延伸複合体 (SEC) がSer2P-phosphorylated Pol IIに吸収されることが観察された.
結論:
- PAF1は,メタゾーンにおけるPol IIのプロモーター近接停止の調節に保存された役割を果たします.
- PAF1は,一時停止したPol IIの放出に影響を与え,潜在的にSECの募集を介して生産的伸長に作用します.
- PAF1は,Pol IIの停止を制御することによって,遺伝子発現の重要なレギュラーとして確立されています.
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