ハイドロキシルラジカルを vivo で検出するための合理的に設計されたアップコンバーションナノプローブ
Zhen Li1, Tao Liang1, Songwei Lv1
1Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Journal of the American Chemical Society
|August 20, 2015
まとめ
研究者は,ヒドロキシルラジカル (•OH) を in vivo で検出するための新しいナノプローブを開発しました. この画期的な発見により,生きた細胞と組織における •OH 濃度のモニタリングに前代未聞の感度がもたらされました.
科学分野:
- 生物医学工学
- 化学生物学
- ナノテクノロジー
背景:
- ヒドロキシルラジカル (•OH) は生理学と病理学において重要な役割を果たします.
- 低濃度で高い反応性があるため, in vivoでOHを検出することは困難です.
研究 の 目的:
- 生体内でのOH検出のための敏感で特異的なナノプローブを開発する.
- 生物学的システムにおけるOHレベルをリアルタイムで監視できるようにする.
主な方法:
- 発光共振エネルギー転送 (LRET) ベースのナノプローブの製造.
- エネルギードナーとしてアップコンバージョンナノ粒子とエネルギー受容体として改変されたアゾ染料 (mOG) とOH認識リガンドを使用します.
- NIR光刺激により 組織に深く浸透し,光傷害を減らす.
主要な成果:
- ナノプローブは,定量化限界1.2フェムトモラーで超高感度を達成しました.
- •OHに対する優れた生物適合性と特異性を証明した.
- 生体細胞と組織におけるOH濃度が成功的にモニタリングされた.
結論:
- 開発されたナノプローブは,OHの in vivo 検出を可能にする最初のものです.
- •OHの生理学的および病理学的役割を理解するための強力なツールを提供します.
- 病気の診断と治療上のモニタリングにおける潜在的な応用
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