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Updated: Apr 5, 2026

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
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Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

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単細胞 伝達 RNA の 配列 解析 に よっ て,まれ な 腸 の 細胞 型 が 発見 さ れ ます

Dominic Grün1,2, Anna Lyubimova1,2, Lennart Kester1,2

  • 1Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences), 3584 CT Utrecht, The Netherlands.

Nature
|August 20, 2015
PubMed
まとめ

私たちは RaceIDという 新しいアルゴリズムを開発し 複合的な単細胞集団の 幹細胞のような希少な細胞タイプを 特定しました この方法は,腸内分泌細胞の新しいマーカーとしてReg4を発見し,新しいサブタイプを明らかにした.

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Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

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Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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