生後1年における早産児および早産児におけるIGF2におけるDNAメチル化の動態:観察研究
Chinthika Piyasena1, Jessy Cartier1, Batbayar Khulan1
1Endocrinology Unit, University/BHF Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK.
Lancet (London, England)
|August 28, 2015
まとめ
胎児は生命の初期に重要なインプリント領域であるIGF2 DMR2およびH19 ICRでDNAメチル化が低下しています. これらのエピジェネティックの変化は,早産と社会的要因と,後の心代謝疾患のリスクを結びつけます.
科学分野:
- エピジェネティクス
- 発達生物学
- 新生児医学
背景:
- 胎児は成人時の心臓代謝疾患のリスクが高い.
- 変化したDNAメチル化 (5-メチルサイトシン,5mC) が潜在的なメカニズムである.
- IGF2 DMR2とH19 ICRにおける5mCの仮説的差異は,早産と出産した乳児の間で見られる.
研究 の 目的:
- IGF2 DMR2とH19 ICRにおけるDNAメチル化の違いを調査する.
- 胎児のメチル化パターンを1年目の胎児と比較する.
- メチル化,成長,社会的要因との関連を調査する.
主な方法:
- 46人の未熟児と40人の未熟児を募りました
- 人体構造と唾液のコルチゾールを測定した
- ピロシーケンスを用いて分析された口腔DNAメチル化 (%5mC); tテストと回帰による統計分析.
主要な成果:
- 妊娠前の乳児は,出生時のIGF2 DMR2の割合が,妊娠後の乳児と比較して5%mC低かった.
- H19 ICRでは,期間に合わせた年齢で5%mCの減少を示した.
- 社会的欠乏は,早期のDMR2で5%mCの減少と関連していた.
結論:
- 5mCの減少は早産児の早期成長障害と相関する.
- IGF2 DMR2とH19 ICRのインプリントは,早産や社会的欠乏などの早期のストレスに敏感である可能性があります.
- これらのエピジェネティック変異は 長期的な健康リスクにつながる可能性があります
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