HNRNPA2B1は,m ((6) A依存核RNA処理イベントの媒介者である

Claudio R Alarcón¹, Hani Goodarzi¹, Hyeseung Lee¹

¹Laboratory of Systems Cancer Biology, Rockefeller University, New York, NY 10065, USA.

Cell

|September 1, 2015

まとめ

RNA結合タンパク質HNRNPA2B1は,N(6) -メチラデノシン (m(6) Aの改変のための核リーダとして作用する. m(6) AライターMETTL3に類似した,プライマリマイクロRNA処理と代替スプライシングに影響を与える.

科学分野:

分子生物学
エピジェネティクス
RNA 生物学

背景:

N(6) -メチラデノシン (m(6) Aは,メッセンジャーRNAで最も一般的な内部変異である.
m(6) Aは核RNAの運命に影響するが,核転写処理を媒介する特定の読者タンパク質は未確認のままである.

研究の目的:

核読タンパク質をm(6) Aマークで識別する.
代替スプライシングとプライマリマイクロRNAの成熟を含む,m(6) A媒介核RNA処理におけるHNRNPA2B1の役割を調査する.

主な方法:

HNRNPA2B1とm(6) A変形RNAの相互作用を評価するためのインビボおよびインビトロ結合測定法.
HNRNPA2B1が代替スプライシングとプライマリマイクロRNA処理に及ぼす影響の分析
プリミRNA処理におけるHNRNPA2B1減少とMETTL3減少の比較

主要な成果:

HNRNPA2B1は,m(6) Aを含むRNAに結合し,m(6) Aコンセンサスモチーフを認識する.
HNRNPA2B1は,METTL3の効果を反映した核転写の代替スプライシングに影響を与えます.
HNRNPA2B1はDGCR8と相互作用し,METTL3の損失に類似する欠陥を引き起こす原始マイクロRNA処理を促進します.

結論:

HNRNPA2B1は,核の m ((6) A リーダとして機能する.
HNRNPA2B1は,初次マイクロRNA処理と代替スプライシングにおけるm(6) Aの役割を媒介する.

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