関連する実験動画

Updated: Apr 4, 2026

Measuring the Kinetics of mRNA Transcription in Single Living Cells
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Measuring the Kinetics of mRNA Transcription in Single Living Cells

Published on: August 25, 2011

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RNAポリメラーゼII転写の開始をリアルタイムで観察する

Furqan M Fazal1, Cong A Meng2, Kenji Murakami3

  • 1Department of Applied Physics, Stanford University, Stanford, California 94305, USA.

Nature
|September 3, 2015
PubMed
まとめ

RNAポリメラーゼIIの転写始動には,大きな前始動複合体 (PIC) が含まれる. 以前から考えられていたこととは対照的に,この複合体は迅速に広範囲のDNAバブルを開き,プロモーターの脱出を可能にします.

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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity09:21

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Transcription Initiation01:47

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RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
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RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
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