内生性タンパク質の可逆性,特異性,活性集積は,熱的ストレスで集合する
Edward W J Wallace1, Jamie L Kear-Scott1, Evgeny V Pilipenko1
1Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57(th) Street, Chicago, IL 60637, USA.
Cell
|September 12, 2015
まとめ
熱ショックにより 酵母細胞のタンパク質の結合が起こりますが これらのタンパク質は 誤った折り畳みではなく 分解せずに活性化されます これは,熱的ストレスへの適応プロセスを示唆しています.
科学分野:
- 細胞生物学
- 生物化学
- ストレス 反応
背景:
- 熱ストレスはタンパク質の誤った折り畳みと結合を誘導し,真核細胞のストレス粒子の形成につながります.
- 熱的ストレス中に集積したタンパク質の運命を理解することは,細胞の生存と適応に不可欠です.
研究 の 目的:
- 熱で誘発されたタンパク質の集積と,その後の解離を,芽生える酵母で定量化する.
- 集積された内生タンパク質が誤った折り畳みで分解されるかどうかを判断する.
- 熱ストレス下での集積タンパク質の機能状態を調査する.
主な方法:
- 芽生えた酵母に安定同位体ラベリングを用いた広範なタンパク質学的研究.
- 熱ショック後のタンパク質の結合と分解の動態の分析
- タンパク質の活性と結合状態の評価
主要な成果:
- >170の内生性タンパク質が 熱ショックから数分以内に 複数の細胞区間に結合することが確認された.
- 熱ショックからの回復中に分解することなく,集積された内生タンパク質が活性化することが判明しました.
- ヘテロトリメリックアミノアシル- tRNA合成酵素複合体は,結合にもかかわらず,不変の忠誠度で活性化し続けました.
結論:
- 熱によって誘発される酵母中のタンパク質の結合は必ずしも誤った折り畳みと分解の兆候ではない.
- 集積されたタンパク質は機能的に重要であり,組み立てと分解を通じて積極的に管理されます.
- このプロセスは,熱的ストレスへの細胞適応のための適応メカニズムを表しています.
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