アンキリンは NOMPC 機械伝導チャネルのゲートに力を送る
Wei Zhang1, Li E Cheng1, Maike Kittelmann2
1Departments of Physiology, Biochemistry, and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
Cell
|September 12, 2015
まとめ
NOMPCチャネルのN端のアンキリンリピート (AR) は,機械的刺激を感知するために不可欠です. これらのARは,チャネルを微小管と結びつけ,触覚を可能にします.
科学分野:
- 細胞生物学
- 神経科学
- バイオ物理学
背景:
- 機械伝達チャネルは 機械的刺激を細胞信号に変換します
- トランジエント受容体ポテンシャル (TRP) チャンネルNOMPCは,ドロソフィラの触覚と聴覚に不可欠です.
- NOMPCのアンキリンリピート (AR) の力伝達における役割は仮説化されているが,完全に理解されていない.
研究 の 目的:
- NOMPCのN端アンキリンリピート (AR) のメカニカルトランスデュークションの機能を調査する.
- NOMPCが機械的な力を感知するタイターメカニズムを解明する.
- NOMPCチャネルゲーティングのためのマイクロチューブル関連の必要性を決定する.
主な方法:
- NOMPCチャネル機能を評価するための in vitroおよびin vivoアッセイ.
- ドロソフィラ内のNOMPCチャネルにおけるARの遺伝子操作
- 他のイオンチャネルにNOMPC ARを転送することによってキメリックチャネルの作成.
主要な成果:
- NOMPCのN末端ARは, in vitroにおけるメカニカゲーション活動と in vivoにおけるメカニカセンシビティに不可欠である.
- ARの重複はマイクロチューブルへの結合長さを変化させ,メカニカル感受性に影響します.
- マイクロチューブルの結合は,NOMPCチャネルゲートに不可欠です.
- NOMPC ARsの移転は,それ以外の無感性のカリウムチャネルに機械感受性を与える.
結論:
- NOMPCのN端 ARは,メカニカル伝導のための力の伝達を媒介する重要な結合として機能する.
- この研究は,NOMPCチャネルゲーティングにおける結合メカニズムの強力な証拠を提供します.
- この発見は,TRPチャネルにおける機械感受性の分子基礎に関する基本的な洞察を提供します.
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