Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

813
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
813
Catalysis02:50

Catalysis

32.4K
The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
32.4K
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

10.3K
10.3K
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

11.5K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
11.5K
Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

12.5K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
12.5K
2° Amines to N-Nitrosamines: Reaction with NaNO201:20

2° Amines to N-Nitrosamines: Reaction with NaNO2

5.8K
Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
5.8K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same author

Role of Polymer-Protein Interactions in the Dynamics of Polymer-Integrated Protein Crystals.

Journal of the American Chemical Society·2026
Same author

Computational Design of a Highly Stable Dicopper Catechol Oxidase.

Journal of the American Chemical Society·2026
Same author

<i>De Novo</i> Design of a Metalloprotein with a Synthetically Inspired Dinuclear Paddlewheel Coordination Motif.

Journal of the American Chemical Society·2025
Same author

Design of a protein scaffold with a selective, Bi-containing heterodinuclear metal coordination motif.

Journal of inorganic biochemistry·2025
Same author

Design of light- and chemically responsive protein assemblies through host-guest interactions.

Chem·2025

関連する実験動画

Updated: Apr 4, 2026

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.8K

窒素酵素触媒における機能的に重要な遭遇複合体の証拠

Cedric P Owens1, Faith E H Katz1, Cole H Carter1

  • 1Department of Chemistry and Biochemistry, University of California, San Diego , La Jolla, California 92039, United States.

Journal of the American Chemical Society
|September 12, 2015
PubMed
まとめ
この要約は機械生成です。

鉄タンパク質 (FeP) とモリブデン鉄タンパク質 (MoFeP) の間の接触複合体は,窒素酵素の触媒に不可欠である. MoFePの静電相互作用を阻害する変異は,FeP-MoFeP結合を阻害することによって,触媒的活動を低下させる.

さらに関連する動画

Synthesis and Performance Characterizations of Transition Metal Single Atom Catalyst for Electrochemical CO2 Reduction
10:57

Synthesis and Performance Characterizations of Transition Metal Single Atom Catalyst for Electrochemical CO2 Reduction

Published on: April 10, 2018

19.4K
Catalytic Reactions at Amine-Stabilized and Ligand-Free Platinum Nanoparticles Supported on Titania During Hydrogenation of Alkenes and Aldehydes
12:08

Catalytic Reactions at Amine-Stabilized and Ligand-Free Platinum Nanoparticles Supported on Titania During Hydrogenation of Alkenes and Aldehydes

Published on: June 24, 2022

4.2K

関連する実験動画

Last Updated: Apr 4, 2026

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.8K
Synthesis and Performance Characterizations of Transition Metal Single Atom Catalyst for Electrochemical CO2 Reduction
10:57

Synthesis and Performance Characterizations of Transition Metal Single Atom Catalyst for Electrochemical CO2 Reduction

Published on: April 10, 2018

19.4K
Catalytic Reactions at Amine-Stabilized and Ligand-Free Platinum Nanoparticles Supported on Titania During Hydrogenation of Alkenes and Aldehydes
12:08

Catalytic Reactions at Amine-Stabilized and Ligand-Free Platinum Nanoparticles Supported on Titania During Hydrogenation of Alkenes and Aldehydes

Published on: June 24, 2022

4.2K

科学分野:

  • 生物化学
  • 酵素学
  • 窒素固定

背景:

  • 窒素酵素は大気中の窒素 (N2) をアンモニア (NH3) に変換するのに不可欠である.
  • このプロセスは,MoFe-タンパク質 (MoFeP) とFe-タンパク質 (FeP) が,ATPに依存した電子移転の方法で相互作用することを含む.
  • タンパク質とタンパク質の相互作用を理解することは,窒素酵素の機能を最適化するための鍵です.

研究 の 目的:

  • 窒素酶触媒における接触複合体の機能的役割を調査する.
  • これらの複合体を安定させる特定の相互作用を特定する.
  • FePとMoFePの電子移転のメカニズムを解明する.

主な方法:

  • 静電相互作用を妨げるために,アゾトバクター・ヴィネランディ MoFePのサイト指向型変異.
  • MoFePの変種の触媒活性を評価する.
  • Thorneley-Loweモデルを用いた運動分析により,結合と電子移転の速度定数を決定する.

主要な成果:

  • 遭遇複合体は,MoFeP βサブユニットの静電相互作用によって安定する.
  • この領域の変異は触媒活性を著しく低下させ,βLys400Gluが最大の効果を示した.
  • βK400E変異は,ATP水解-電子移転結合に影響を与えることなく,FeP-MoFeP結合速度の定数を5倍に低下させた.

結論:

  • 遭遇複合体は,窒素酶触媒に機能的な役割を果たす.
  • FePは最初,MoFeP β亜単体の表面に遭遇複合体を形成する.
  • この相互作用は,α-インターフェイスでATP活性化,電子移転能力の複合体の形成の前提条件である.