アミロイド型トランスレーション抑制体の調節された形成がゲメトゲネシスを制御する

Luke E Berchowitz¹, Greg Kabachinski², Margaret R Walker¹

¹David H. Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Cell

|September 29, 2015

まとめ

酵母タンパク質Rim4は,ゲメトゲネシス中の遺伝子翻訳を制御するために,アミロイドのような集合体を形成します. この調節された結合と分解プロセスは細胞の発達に不可欠です

科学分野:

分子生物学
細胞生物学
生物化学

背景:

ガメトゲネシスには遺伝子発現の正確な制御が必要です特にトランスレーションの際にです
RNA結合タンパク質Rim4は酵母におけるmRNA翻訳を調節し,CLB3のようなメイオシスに不可欠な遺伝子に影響を与えます.
アミロイドは一般的に病気と関連していますが,その生理学的役割はますます認識されています.

研究の目的:

イーストのゲメトゲネシス中にRim4が翻訳を調節するメカニズムを調査する.
リム4の構造,特にアグリゲーションが,その機能と関連しているかどうかを判断する.
細胞プロセスにおけるアミロイドのようなタンパク質構造の生理学的関連性を探求する.

主な方法:

酵母遺伝学と分子生物学の技術です
リム4構造を視覚化するためのタンパク質集積アッセイ
変換活動とタンパク質の分解を評価するための生化学的分析

主要な成果:

リム4はアミロイド状の集合体を形成し,この集合体は翻訳的に抑圧的です.
飢えによって誘発される.
リム4集積物は,メオシスIIの初期に急速に分解され,翻訳が可能になる.

結論:

細胞は,Rim4によって形成されるようなアミロイド型のタンパク質を,ゲメトゲネシスの機能的調節剤として利用する.
Rim4アグレガートの形成とクリアランスは,メッセージ特有のトランスレーション制御のための新しいメカニズムを表します.
この研究は,基本的な生物学的プロセスにおけるアミロイドのような構造の非病理的な役割を強調しています.

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