イオン移動質量スペクトロメトリーを用いた炭水化物アノマーの特定

J Hofmann^1,2, H S Hahm^3,4, P H Seeberger^3,4

¹Fritz Haber Institute of the Max Planck Society, Faradayweg 4-6, 14195 Berlin, Germany.

Nature

|September 30, 2015

まとめ

離子移動質量スペクトロメトリーは,複雑な炭水化物の同位体と,炭水化物の分析における重要な進歩を区別することができる. この技術は小さなサンプルで微小な同位体さえも正確に識別し分野を革命的に変えました

科学分野:

生物化学
分析化学
グライコバイオロジー

背景:

炭水化物は複雑な構造を持つ重要な生物学的ポリマーであり,ペプチドやオリゴヌクレオチドと比較して特徴づけることは困難です.
NMRスペクトロスコーピーのような伝統的な方法は,大量のサンプルを必要とし,同位体検出に苦労しますが,質量スペクトロメトリーは立体同位体を区別することはできません.

研究の目的:

イオン移動質量スペクトロメトリー (IM-MS) が炭水化物の結合同位体とステレオ同位体を明確に識別する能力を実証する.
複雑な炭水化物に対する敏感で迅速な分析ツールとして IM-MSを確立する.

主な方法:

イオン移動質量スペクトロメトリを用いた6つの合成炭水化物イソマーの分析.
質量,電荷,サイズ,形状に基づいて分子を分離し,同位体と区別する.

主要な成果:

イオン移動質量スペクトロメトリーは,炭水化物の結合同位体とステレオ同位体を成功裏に明確に特定しました.
この方法は,0.1%の濃度までマイナーなイソメアを定量化する能力を持つ共存イソメアを検出しました.
分析は迅速で,誘導法を必要とせず,最小のサンプル量を使用しました.

結論:

イオン移動質量スペクトロメトリは,複雑な炭水化物の総合的な分析のための強力で効果的なツールです.
この技術は,ペプチド組立に対するHPLCの効果に類似して,炭水化物の合成に著しく影響を与える可能性があります.

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