トランスフォーメーションサプレッサー活性を持つras関連遺伝子
H Kitayama1, Y Sugimoto, T Matsuzaki
1Laboratory of Molecular Oncology, Tsukuba Life Science Center, Ibaraki, Japan.
Cell
|January 13, 1989
まとめ
研究者は,癌細胞の成長を抑制し,rasタンパク質との類似性を共有するKrev-1遺伝子を特定しました. この遺伝子は種全体に存在し,広く発現しており,組織調節における重要な役割を果たしていることを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- NIH/3T3細胞のキルステン・サルコマウイルスの変異は,悪性現象型につながります.
- この変異を逆転させることができる遺伝子を特定することは,がんの生物学を理解するために不可欠です.
研究 の 目的:
- NIH/3T3細胞の変形フェノタイプを逆転させる能力を持つcDNAクローンを分離し,特徴づけること.
- 特定された遺伝子の機能と進化的保存を調査する.
主な方法:
- 人間の線維芽細胞からcDNA発現ライブラリを分離した.
- NIH/3T3細胞をKrev-1cDNAクローンで感染させる.
- mRNAレベルとフェノタイプ抑制の分析.
- 既知のタンパク質とアミノ酸配列の比較.
- サザン・ブレット分析は,他の種における同類の配列を検出するためのものです.
主要な成果:
- 1.8kbのcDNAクローンであるKrev-1が分離され,リバータント誘発活性を示した.
- Krev-1トランスフェクタントは,特定のmRNAレベルと変異したフェノタイプの抑制の間の相関を示しました.
- Krev-1のcDNAは,21,000ダルトンのタンパク質をコードし, rasタンパク質と約50%のアミノ酸同一性を持っています.
- ホモログなKrev-1配列はマウス,ネズミ,鶏のDNAで検出され,ネズミの臓器に広く発現した.
結論:
- Krev-1遺伝子は,様々な組織で重要な役割を果たしています.
- Krev-1は,特定の細胞タイプにおける細胞成長の負の調節に関与している可能性があります.
- rasタンパク質との構造的類似性は,成長制御に関連する細胞信号伝達経路における潜在的な役割を示唆しています.
関連する概念動画
02:38The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
02:38The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...