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関連する概念動画

GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

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GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
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Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
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In order to make good decisions, we use our knowledge and our reasoning. Often, this knowledge and reasoning is sound and solid. However, sometimes, we are swayed by biases or by others manipulating a situation. For example, let’s say you and three friends wanted to rent a house and had a combined target budget of $1,600. The realtor shows you only very run-down houses for $1,600 and then shows you a very nice house for $2,000. Might you ask each person to pay more in rent to get the...
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Anchoring Junctions01:03

Anchoring Junctions

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Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
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Lipids as Anchors01:32

Lipids as Anchors

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In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
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Complement System01:27

Complement System

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The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
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関連する実験動画

Updated: Feb 8, 2026

GPI Anchoring of Proteins in the ER Membrane
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酵母HAP1アクティベーターの機能的解剖と配列

K Pfeifer1, K S Kim, S Kogan

  • 1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

Cell
|January 27, 1989
PubMed
まとめ

研究者は酵母活性化剤HAP1を解剖し,標的部位の認識に不可欠な亜鉛指を持つDNA結合ドメインを特定しました. 独立した領域がヘム誘導を媒介し,カルボキシル末端が転写活性化を誘導する.

科学分野:

  • 分子生物学は分子生物学である.
  • イースト遺伝学 イースト遺伝学
  • 転写因子 トランスクリプション・ファクター

背景:

  • イーストアクティベーターHAP1は,遺伝子発現の重要なレギュレータです.
  • そのDNA結合と規制メカニズムを理解することは,細胞のプロセスを解読する上で極めて重要です.

研究 の 目的:

  • 1483-残留酵母活性化剤HAP1.1を機能的に解剖する.
  • DNA結合,ヘム誘導,および転写活性化に責任を負う特定のドメインを特定する.

主な方法:

  • HAP1内の特定の残留物を変化させるためのサイト指向型変異性.
  • HAP1の標的部位との相互作用 (UAS1とCYC7) を評価するためのDNA結合アッセイ.
  • 変異研究を通じて,HAP1の機能ドメインの分析.

主要な成果:

  • システインに富んだ亜鉛指を媒介する単一のDNA結合ドメイン (残留1-148) がUAS1とCYC7.7への結合を媒介する.
  • 亜鉛指内または周りの特定の変異は,UAS1またはCYC7への結合を廃止し,シーケンス固有の認識を示します.
  • 独特の領域 (残留物245-445) は,DNA結合ドメインを覆い,ヘム誘導に関与し,ヘムはこの効果を相殺する.

さらに関連する動画

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関連する実験動画

Last Updated: Feb 8, 2026

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01:29

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01:25

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  • 高酸性カルボキシル末端は,HAP1媒介の転写活性化に不可欠である.

    • 結論:

    • HAP1のDNA結合特異性は,その亜鉛指と隣接する残留物によって決定されます.
    • ヘム誘導は,金属結合重複領域によって調節されるマスクメカニズムを含む.
    • カーボキシル末端は,HAP1.1の主要な転写活性化ドメインとして機能する.