酵母フェロモン反応における構成変異体:遺伝子産物の秩序付けられた機能

D Blinder¹, S Bouvier, D D Jenness

¹Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.

Cell

|February 10, 1989

まとめ

研究者は,常に活発なフェロモン応答経路を持つ酵母変異体を特定しました. この研究は,酵母細胞分裂とシグナル伝達におけるSCG1およびSTE4遺伝子の役割に関する新しい洞察を明らかにしています.

科学分野:

分子生物学は分子生物学である.
細胞生物学細胞生物学
イースト遺伝学イースト遺伝学

背景:

アルファファクターフェロモンは,酵母細胞分裂の調節に不可欠です.
フェロモンの反応経路を理解することは,酵母細胞サイクル制御の鍵です.

研究の目的:

構成フェロモン応答を持つ酵母変異体を分離する方法を開発する.
酵母フェロモンのシグナル伝達経路に関与する遺伝子を特定するために.

主な方法:

一般的なスクリーニング方法を使用して酵母変異体の分離.
STE4とSCG1.1を含むダブルミュータントの遺伝子解析

主要な成果:

支配的なSTE4アレルと後退性SCG1変異が特定されました.
SCG1とSTE4は,それぞれGαとGβ同類体をコードする.
STE4はSCG1より下流に機能するが,経路ではSTE5より上流に機能する.

結論:

この研究は,酵母フェロモン反応を調査する方法を提供しています.
酵母シグナル伝達におけるSCG1とSTE4の連続的な機能を明らかにする.

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