水交換率を変えることで,亜鉛 (II) 反応性MRIコントラスト剤の感度を増大させる

Jing Yu¹, André F Martins¹, Christian Preihs²

¹Department of Chemistry, University of Texas at Dallas , P.O. Box 830668, Richardson, Texas 75083, United States.

Journal of the American Chemical Society

|October 15, 2015

まとめ

新しいMRIコントラスト剤は,亜鉛検出の感度を改善するために開発されました. これらの薬剤は以前のバージョンと比較して信号が3倍に増加し,臓中の亜鉛の放出をインビボ画像化することが可能である.

科学分野:

医療用画像検査
生物化学
材料科学

背景:

以前,Zn (II) に敏感なMRIコントラスト剤は,水交換速度が遅いため制限に直面しました.
水の交換を最適化することはMRI検出の感度を高めるために重要です.

研究の目的:

新しいMRIコントラスト剤を設計し,Zn (II) の検出のために水交換特性を改善する.
MRIの感度を増やすために,縦方向のリラキシビティ (r1) を強化する.

主な方法:

GdDOTA-diBPENに基づいた新しいセンサー構造の合理的な設計.
水交換特性と運動安定性の特徴
Zn (II) とヒト血清アルブミン (HSA) との結合時の縦断緩解度 (r1) の測定

主要な成果:

新しいセンサーは精密調整された水交換特性を示しています
2つの最適化されたセンサーは,Zn (II) /HSAで約50 mM (-1) s (-1) の高い縦緩度 (r1) を達成した.
元のGdDOTA- diBPENの3倍に増加した.
グルコース刺激によるインスリン分泌中にマウスの臓からZn (II) の放出が成功しました.

結論:

構造変更により,MRIコントラスト剤の水交換率と放緩性が著しく改善されます.
開発された高リラクシビティ剤は,生物学的関連性のあるZn (II) ダイナミクスのインビボ画像化に有効である.

さらに関連する動画

14:01

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

Published on: July 22, 2011

15.1K

13:21

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Published on: July 21, 2011

15.5K

関連する概念動画

01:26

EDTA: Auxiliary Complexing Reagents

1.6K

EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...

1.6K

01:19

Masking and Demasking Agents

4.0K

EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...

4.0K

01:24

Magnetic Resonance Imaging

10.3K

Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...

10.3K

01:14

Imaging Studies I: CT and MRI

1.2K

Introduction: MRI and CT scans are crucial advancements in medical imaging techniques, playing a vital role in diagnosing conditions related to the gastrointestinal (GI) system. Each scan serves distinct purposes, targets specific areas, and requires unique nursing duties.
Description of the Procedures
Computed Tomography (CT) scan:
Computed Tomography (CT) scans use X-ray technology to generate detailed images of bones, organs, and tissues. During the scan, the patient lies on a moving table...

1.2K

00:56

Extraction: Advanced Methods

1.3K

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...

1.3K

01:17

Ion Exchange

1.5K

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...