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A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
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膜幾何学に敏感な分子の構造の研究のための球形ナノ粒子のサポートされた脂質二層

Riqiang Fu1, Richard L Gill2, Edward Y Kim3

  • 1National High Magnetic Field Laboratory, Tallahassee, Florida 32310, United States.

Journal of the American Chemical Society
|October 22, 2015
PubMed
まとめ

スポVMタンパク質と曲線膜の相互作用は,球形ナノ粒子のサポートされた脂質二層 (SSLB) を使用して研究されました. この研究は,SLSBが膜幾何学に敏感な分子を分析するのに有効であることを示しています.

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Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
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