酵母HO遺伝子の細胞サイクル制御に関与するDNA結合因子の特定

B J Andrews¹, I Herskowitz

¹Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.

Cell

|April 7, 1989

まとめ

HO遺伝子の細胞サイクル調節には,細胞サイクルボックス因子 (CCBF) が関与しています. SWI4およびSWI6遺伝子は,G1段階におけるCCBF活動とHO遺伝子発現に不可欠である.

科学分野:

分子生物学は分子生物学である.
イースト遺伝学イースト遺伝学
細胞サイクル規制について

背景:

HO遺伝子の転写は,複数のトランス作用因子によって厳密に規制されています.
特定の調節器と細胞サイクルボックス (CCB) 要素は,G1段階の終わりにHO発現を制御します.

研究の目的:

細胞サイクルボックス要素に結合する因子を特定するために.
CCBFプロモーター複合体の形成とHO遺伝子発現におけるSWI遺伝子の役割を決定する.

主な方法:

タンパク質-DNA相互作用を検出するためのゲル遅延アッセイ.
DNaseの足跡は,因子結合部位をマップする.
遺伝子発現を評価するためのインビトロおよびインビボアッセイ.

主要な成果:

新しい因子である細胞サイクルボックス因子 (CCBF) が特定され,CCB要素と結合する.
SWI4とSWI6遺伝子は,CCBFプロモーター複合体の形成に必要である.
SWI4とSWI6は,CCB媒介によるHO遺伝子発現に必要な唯一のSWI遺伝子である.

結論:

CCBFは,細胞サイクルに依存するHO遺伝子転写の重要な調節体である.
SWI4とSWI6は,この細胞サイクル調節を媒介する上で,特定の重要な役割を果たしています.

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