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Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
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2,3,5-トリフルオロチロシン置換リボヌクレオチド還元酵素における反転電子移転は,触媒サイクルを完了する

Kanchana R Ravichandran, Ellen C Minnihan, Yifeng Wei

  • 1Department of Chemistry and Chemical Biology, Harvard University , 12 Oxford Street, Cambridge, Massachusetts 02138, United States.

Journal of the American Chemical Society
|October 23, 2015
PubMed
まとめ

この研究では,Escherichia coliのリボヌクレオチド還元酵素が,核酸二酸化塩素をデオキシヌクレオチドに変換する経路をどのように使用しているかが明らかになりました. 修正されたチロシン (F3Y) を導入することで,主要な中間基の観察が可能になり,酵素の触媒サイクルが明確になりました.

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