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Updated: Mar 31, 2026

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

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マスクロマトグラムの二次元分析による非正規のRNAの双方向的直接シーケンシング

Anders Björkbom1,2,3, Victor S Lelyveld1,2, Shenglong Zhang1

  • 1Howard Hughes Medical Institute , Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, United States.

Journal of the American Chemical Society
|October 27, 2015
PubMed
まとめ

この研究は,RNAシーケンシングのための複雑な質量スペクトロメトリ (MS) のデータを簡素化します. 液体クロマトグラフィーとモンテカルロアルゴリズムを用いた新しい方法は,改変された核酸でRNAの堅固なデノボシーケンシングを可能にします.

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