遺伝子調節要素におけるRNAによる転写因子捕獲
Alla A Sigova1, Brian J Abraham1, Xiong Ji1
1Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Science (New York, N.Y.)
|October 31, 2015
まとめ
転写因子 (TFs) は,遺伝子を制御するためにDNAを結合します. この研究では,調節性DNA要素から生成されるRNAは,TF結合を安定させ,遺伝子発現のためのフィードバックループを作成することを示しています.
科学分野:
- 分子生物学
- 遺伝子規制
- エピジェネティクス
背景:
- 転写因子 (TF) は,特定のDNA配列に結合することによって,遺伝子調節に不可欠です.
- プロモーターを含む規制要素は,RNAトランスクリプトを生成する.
- いくつかのTFはRNAに結合し,TF機能におけるRNAの潜在的な役割を示唆する.
研究 の 目的:
- 転写因子占有率を維持する規制要素から転写されたRNAの役割を調査する.
- RNA結合が遺伝子調節部位でのTF結合の安定性に寄与するかどうかを判断する.
- RNAとTFの結合を含むポジティブフィードバックメカニズムの可能性を調査する.
主な方法:
- DNAとRNAに結合する転写因子Yin-Yang 1 (YY1) の全ゲノム分析
- YY1占有率への影響を評価するために,規制要素からの転写を操作する.
- YY1結合への影響を観察するために,遺伝子調節要素にRNAの実験的結合.
主要な成果:
- 転写因子YY1は,ゲノム全体でDNAの調節要素と関連するRNA種の両方に結合する.
- 規制要素からの転写の減少は,YY1占有率の減少につながります.
- RNAの人工結合は,これらの規制要素のYY1占有率を高めます.
結論:
- YY1のような特定の転写因子の安定結合に寄与する.
- 規制要素の転写は,TFの安定性を高め,堅固な遺伝子発現プログラムを促進する,ポジティブなフィードバックループを確立する.
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