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Updated: Mar 29, 2026

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
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Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

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単一のアミノ酸は,フラビン依存デハロゲネーゼとニトロリダクトーゼの間で切り替わります

Arnab Mukherjee1, Steven E Rokita1

  • 1Department of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.

Journal of the American Chemical Society
|December 1, 2015
PubMed
まとめ

フラボタンパク質の単一の変異は,その機能をデハロゲネーゼからニトロリダクトーゼに変えることができます. この発見は,酵素の進化と機能を理解することによって,新しい触媒を開発するのに役立ちます.

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Electrophilic Aromatic Substitution: Nitration of Benzene

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All meta-directing substituents are deactivating groups. These substituents withdraw electrons from the aromatic ring, making the ring less reactive toward electrophilic substitution. For example, the nitration of nitrobenzene is 100,000 times slower than that of benzene because of the deactivating effect of the nitro group. The first step in an electrophilic aromatic substitution is the addition of an electrophile to form a resonance-stabilized carbocation. The energy diagrams for...
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The reaction begins with an attack of the nucleophile on the carbon that holds the leaving group. This results in the delocalization of the π electrons over the ring carbons. The resonance interaction between...
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