関連する実験動画

Updated: Mar 29, 2026

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
11:37

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

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精密性が向上した合理的に設計されたCas9核酸

Ian M Slaymaker1, Linyi Gao2, Bernd Zetsche1

  • 1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Science (New York, N.Y.)
|December 3, 2015
PubMed
まとめ

強化特異性Cas9 (eSpCas9) と呼ばれる Cas9の改造された変種は,ゲノム編集における意図しないDNA分裂を大幅に減少させます. これらの改良されたCas9ツールは より安全な遺伝子編集アプリケーションのために 精密なターゲティングを維持します

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