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関連する概念動画

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Overview of Electron Microscopy01:25

Overview of Electron Microscopy

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Scanning Electron Microscopy01:07

Scanning Electron Microscopy

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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Updated: May 1, 2026

High Content Screening in Neurodegenerative Diseases
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High Content Screening in Neurodegenerative Diseases

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顕微鏡による高濃度スクリーニング

Michael Boutros1, Florian Heigwer2, Christina Laufer2

  • 1Division Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Department of Cell and Molecular Biology, Heidelberg University, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany; German Cancer Consortium (DKTK), 69120 Heidelberg, Germany.

Cell
|December 7, 2015
PubMed
まとめ
この要約は機械生成です。

画像ベースのスクリーニングは 波動と組み合わせて 生物学的プロセスに 強力な洞察力を提供します 画像と分析の進歩により CRISPRの応用を含む大規模な研究が加速しています

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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

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Using High Content Imaging to Quantify Target Engagement in Adherent Cells
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関連する実験動画

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High Content Screening in Neurodegenerative Diseases
13:32

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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
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Using High Content Imaging to Quantify Target Engagement in Adherent Cells
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科学分野:

  • * 細胞と生物の生物学
  • * 高濃度スクリーニングと画像検査
  • * ゲノム工学と機能的ゲノミクス

背景:

  • * 画像ベースのスクリーニングは,細胞と生物のフェノタイプを定量化します.
  • * RNAの干渉や小分子などの乱れは,体系的な生物学的洞察を強化します.
  • * 適用範囲は,タンパク質の局所化,がんの脆弱性,生物の表型などです.

研究 の 目的:

  • * 画像ベースのスクリーニングの最先端をレビューする.
  • * 実験方法と画像分析方法の定義
  • CRISPR/Cas9を含む課題と将来の方向性を議論する.

主な方法:

  • * 画像ベースのスクリーニングで,様々な変異 (RNAi,小分子,変異) を利用する.
  • * 大規模なスクリーンのための高度な画像と画像分析方法論を使用しています.
  • CRISPR/Cas9ゲノム工学の統合を強調しています.

主要な成果:

  • * 画像ベースのスクリーニングは,生物学的プロセスに対する体系的な洞察を提供します.
  • * 最近の進歩により,大規模な乱射画面が加速しています.
  • * 技術の現在の能力と限界について議論します.

結論:

  • * 画像ベースのスクリーニングは生物学的発見のための強力なツールです.
  • * 画像と分析の継続的な進歩は極めて重要です.
  • * CRISPR/Cas9の統合は,拡張されたアプリケーションを約束しています.