v-myb腫瘍遺伝子の転写活性化と,その細胞の祖先であるc-mybによる転写活性化

K Weston¹, J M Bishop

¹Department of Microbiology and Immunology, University of California, San Francisco 94143-0552.

Cell

|July 14, 1989

まとめ

v-myb がん基因は,v-myb がん基因が,v-myb がん基因が,その

科学分野:

分子生物学は分子生物学である.
腫瘍学腫瘍学

背景:

v-myb腫瘍遺伝子は,細胞の成長と分化を調節する核タンパク質をコードする.
その細胞の対称はc-mybである.

研究の目的:

転写活性化に責任を負うv-mybドメインを識別する.
遺伝子調節におけるこのドメインの役割を調査する.

主な方法:

v-mybを GAL4 DNA結合ドメインと結びつける融合タンパク質の構築.
GAL4またはmyb結合部位を持つレポーター遺伝子を用いて転写活性化の評価.
v-mybアクティベータードメインに削除を導入する.

主要な成果:

v-myb内の特定のドメイン (残留204-254) は,転写活性化に必要かつ十分であると特定されました.
融合タンパク質は,GAL4結合部位からの転写を活性化しました.
健全なv-mybおよびc-mybタンパク質は,myb結合部位を通じた転写を活性化しました.
アクティベータードメインの削除により,転写刺激は廃止された.

結論:

v-myb腫瘍遺伝子は,特異な転写活性化ドメインを有する.
このドメインは,遺伝子発現を調節する腫瘍遺伝子の機能にとって極めて重要です.
この領域を理解することで,腫瘍遺伝子を駆動する細胞の成長と分化に関する知識が進歩します.

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