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関連する概念動画

Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Operon Model01:23

Operon Model

The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...

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関連する実験動画

Updated: Jul 8, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

deoR抑制器が自然オペレータ部位と結合すると,単一および二重ループが形成されます.

M Amouyal1, L Mortensen, H Buc

  • 1Unité de Physicochimie des Macromolécules Biologiques, URA1149 du CNRS, Institut Pasteur, Paris, France.

Cell
|August 11, 1989
PubMed
まとめ

deoR抑制器は,deo操作を制御するためにDNAループを形成します. 電子顕微鏡でこれらのループを可視化し,オペレータの部位で協同結合による遺伝子抑制におけるその役割を確認した.

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオフィジックス 生物物理学

背景:

  • deoオペロンのin vivo抑制は, deoR抑制体とDNAループ形成と関連しています.
  • DNAループは,長距離の遺伝子発現を調節する重要なメカニズムです.

研究 の 目的:

  • DNAループの形成における deoR 抑制体の役割を調査する.
  • deoR抑制器によって媒介されるDNAループの構造を視覚化および特徴づけること.
  • デオオペロンのインビボ抑制とDNAループ形成を相関させるため.

主な方法:

  • 電子顕微鏡を用いて,DNA-タンパク質複合体を視覚化しました.
  • deoオペロンオペレータを含むDNA断片は,オリゴメリック deoR抑制剤で化されました.
  • ループのサイズを測定し,分析しました.

主要な成果:

  • deoR抑制器は,2人のオペレーターと結合すると,特定のサイズ (280,600,880 bp) の単一のDNAループを形成しました.
  • 3つのオペレーターに同時に結合すると,ダブルループ (280bp + 600bp) が形成されます.
  • 観察されたループ形成は,in vivoで見られた長距離抑制効果と一致しています.

さらに関連する動画

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

関連する実験動画

Last Updated: Jul 8, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

結論:

  • deoR抑制器によるDNAループ形成は, deoオペレオンを調節する直接的なメカニズムです.
  • deoR抑制器を3つのオペレーターサイトすべてに協力的に結合することは,効率的な抑制に不可欠です.
  • 電子顕微鏡は,遠隔遺伝子調節の分子基礎の視覚的証拠を提供します.