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Updated: Mar 27, 2026

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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CIRCLE-Seq for Interrogation of Off-Target Gene Editing

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高精度CRISPR-Cas9核酸で,ゲノム全体で検出可能なオフターゲット効果はありません

Benjamin P Kleinstiver1,2, Vikram Pattanayak1,2, Michelle S Prew1

  • 1Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.

Nature
|January 7, 2016
PubMed
まとめ

新しい高精度CRISPR-Cas9変種であるSpCas9-HF1は,ゲノム編集における望ましくない非標的変異を大幅に減少させます. この高精度なツールは 標的の活動を維持し 研究や治療の応用に より安全な代替手段を提供します

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