ポリA結合タンパク質は,ポリA短縮と60Sリボソームサブユニットに依存した翻訳開始のために必要です
1Department of Biochemistry, Stanford University Medical Center, California 94305.
Cell
|September 8, 1989
まとめ
ポリア) 結合タンパク質 (PAB) は,酵母における翻訳開始に不可欠である. サプレッサー変異は,60Sリボソームサブユニットが,効率的なmRNA翻訳のためのこのPAB要件を媒介することを明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
- タンパク質合成 タンパク質合成
背景:
- エッセンシャルなポリア) 結合タンパク質 (PAB) は,mRNAの調節に重要な役割を果たします.
- S. cerevisiaeにおけるPABの減少は,翻訳の開始を阻害し,ポリ (A) 尾を短くする.
研究 の 目的:
- 翻訳開始にPABが必要になるメカニズムを調査する.
- PAB機能の喪失を抑制する遺伝的要因を特定する.
主な方法:
- 温度感受性変異 (pab1-F364L) とプロモーター不活性化を使用して,S. cerevisiae.でPABを枯渇させる.
- 逆転分析を行い,冷たい感受性抑制子変異 (spb1-spb7) を分離する.
- 抑制器変異が翻訳開始,ポリ (A) 尾の長さ,およびリボソームサブユニット組成に及ぼす影響を分析する.
主要な成果:
- PABの消去を抑制する7つのエキストラジェニックの冷たい感受性変異 (spb1-spb7) が特定されました.
- これらの変異により,PABが存在しない場合でも,ポリー (A) 尾の長さを変化させずに翻訳を開始することができます.
- サプレッサー変異は60Sリボソームサブユニットの量に影響することが判明し,SPB2はリボソームタンパク質L46.6をコードする.
結論:
- 60Sリボソームサブユニットは,翻訳開始時にPABの要求を媒介することに関与しています.
- この相互作用は,無傷のポリ(A) + mRNAの効率的な翻訳をin vivoで保証します.
- この経路を理解すると,mRNAの品質管理と翻訳的調節に関する洞察が得られます.
関連する概念動画
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Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
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Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
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Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Initiation of Translation
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Initiation of Translation
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
Translation in Prokaryotes
Prokaryote translation is a complex, highly coordinated process that converts genetic information from mRNA into functional proteins. It involves three stages: initiation, elongation, and termination, each facilitated by specific molecular components.Initiation of TranslationThe process begins with the assembly of the ribosomal subunits and initiation factors on the mRNA. In bacteria, the 30S ribosomal subunit recognizes the Shine-Dalgarno sequence in the mRNA, a conserved region upstream of...


