関連する実験動画
Updated: Mar 27, 2026

12:48
The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
12.6K
E. coli のタンパク質発現に対するコドンの影響は,mRNA レベルと相関する.
Grégory Boël1,2,3, Reka Letso1,2, Helen Neely1,2
1Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, NY 10027, USA.
Nature
|January 14, 2016
まとめ
遺伝子コードの変異は,mRNAの分解とタンパク質の延長運動を調節することによって,タンパク質発現に影響を与えます. コドンの含有量は,使用頻度だけでなく,Escherichia coliの遺伝子発現レベルに影響します.
科学分野:
- 分子生物学
- 遺伝学
- 生物化学
背景:
- 遺伝子コードの退化により 複数のDNA配列が 同じタンパク質をコードし 遺伝子発現に影響を与えます
- 同義的なコドンがタンパク質とmRNAレベルに影響を与える正確なメカニズムは,まだ完全に理解されていません.
研究 の 目的:
- タンパク質発現レベルに影響を与える配列の特徴,特にコドン含有量を調査する.
- 遺伝子発現に対するコドン影響のメトリックを開発し,それをmRNA折り畳みパラメータと比較する.
主な方法:
- 菌糸体T7ポリメラーゼを用いた6348件の実験の分析
- ロジスティック回帰を適用して新しいコドン影響メトリックを導き出す.
- コドンの影響とゲノムコドン使用およびmRNA折り畳みパラメータの比較
主要な成果:
- 新しいコドン影響メトリックは,コドン使用と弱い相関を示したが,タンパク質とmRNA濃度と寿命と強い相関を示した.
- コドン含有量は,最初の ~ 16 コドンを除いて,mRNA 折り畳みよりもタンパク質発現の強い決定因子でした.
- 再設計された遺伝子は同様の転写を示したが,インビトロ翻訳は強化され,ネイティブの非効率的な配列はインビボのmRNAレベルが低下した.
結論:
- コドンの含有量は,タンパク質の延長とmRNAの分解のバランスに影響することで,タンパク質発現を著しく調節する.
- この運動競争は,エシェリキア・コライの生理学と転移の調節の重要な側面です.
関連する概念動画
Leaky Scanning
5.9K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.9K
Ribosome Profiling
4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
Improving Translational Accuracy
15.4K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
15.4K
From DNA to Protein
24.4K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
24.4K
What is Gene Expression?
12.2K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
12.2K
What is Gene Expression?
199.3K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
199.3K

