酵母 の 相互作用 に よっ て,酵母 から 人間 へ の ネットワーク の 進化 の 原則 が 明らか に なり ます
Tommy V Vo1, Jishnu Das2, Michael J Meyer3
1Department of Biological Statistics and Computational Biology, Cornell University, Ithaca, NY 14853, USA; Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY 14853, USA; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Cell
|January 16, 2016
まとめ
FissionNetは,分裂酵母 (S. pombe) の何千もの新しいタンパク質の相互作用を明らかにし,新しい生物学的経路と酵母と人間のタンパク質の驚くべき進化的つながりを明らかにしています.
科学分野:
- プロテオミクス
- 酵母遺伝学
- システム生物学
背景:
- タンパク質の相互作用を理解することは 細胞の機能を解読するのに不可欠です
- 種間の比較インタラクタミクスは,進化の保存と分岐を明らかにすることができます.
- 分裂酵母 (S. pombe) のようなモデル生物は,基本的な生物学的プロセスを研究するために不可欠です.
研究 の 目的:
- S. pombe のための包括的なプロテオーム幅のバイナリタンパク質相互作用マップを構築する.
- 種別タンパク質の相互作用ネットワークの再配線を調査する.
- タンパク質とタンパク質の相互作用の 進化的保存を研究する
主な方法:
- S. pombeの高品質のバイナリタンパク質相互作用データセットであるFissionNetの生成.
- 検定の感度と特異性を考慮したネットワーク比較の枠組みの開発.
- 種間相互作用マッピングと遺伝子複製効果の分析
主要な成果:
- FissionNetは2,278の高品質のS. pombeの相互作用を含んでおり, ~50%は新しいものです.
- 遺伝子サイレンスとmRNA前スプライシングにおける新しい相互作用を発見した.
- S. pombeの相互作用は,S. cerevisiaeよりもヒトでより良く保存されている.
結論:
- タンパク質の機能と進化を理解するための貴重なリソースです.
- 比較インタラクタミクスは,相互作用するタンパク質の重要な進化的再配線と共同進化を強調しています.
- この発見は,ヒトの病気を研究するためにモデル生物を使用する際の影響を及ぼします.
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