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Viruses with RNA Genomes01:29

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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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二重RNA-seqは,宿主-病原体相互作用における非コーディングRNA機能を明らかにする.

Alexander J Westermann1, Konrad U Förstner1,2, Fabian Amman3,4

  • 1University of Würzburg, RNA Biology Group, Institute for Molecular Infection Biology, Josef-Schneider-Straße 2/D15, D-97080 Würzburg, Germany.

Nature
|January 21, 2016
PubMed
まとめ

研究者は細菌の小さなRNA PinTを発見し 感染中にサルモネラ菌の毒性を制御しています この発見は病原体と宿主との相互作用と 細菌の遺伝子調節に関する新しい洞察をもたらします

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科学分野:

  • 微生物学
  • 分子生物学
  • ゲノミクス

背景:

  • 細菌の小さなRNA (sRNA) は遺伝子調節において重要な役割を果たしますが,病原性におけるその機能はしばしば不明です.
  • 標準的な毒性測定では,宿主-病原体の相互作用中に多くの細菌sRNAの影響を明らかにする感度が欠けている.

研究 の 目的:

  • サルモネラ・エンテリカ・セロヴァー・タイフィムリウム感染症における細菌の小RNAの調節作用を調査する.
  • 病原体と宿主の両方の同時RNA発現をプロファイルし,新しい調節メカニズムを明らかにする.

主な方法:

  • 感染中に病原体と宿主遺伝子の発現を同時に分析するために,二重RNA配列化 (RNA-seq) のアプローチを使用した.
  • バクテリアのsRNAを宿主細胞経路にリンクするために,種間相関分析を行った.

主要な成果:

  • 細菌の侵入と細胞内生存遺伝子を一時的に制御するPhoP活性化小RNA PinTを特定しました
  • JAK- STATシグナル伝達経路を含む宿主細胞のコード化および非コード化RNAトランスクリプトをPintが有意に変化させることが示された.
  • 宿主の長い非コーディングRNAに感染特有の変異を発見した.

結論:

  • 双重RNA-seqアプローチは,細胞内細菌の病原体とそれらの宿主を物理的に分離せずに研究するための敏感な方法を提供します.
  • PinTのような細菌のリボレギュレータは,宿主細胞の遺伝子発現と信号伝達経路に大きな影響を与えます.
  • この研究は 病原体遺伝子の隠された機能を発見し ホスト-病原体ダイナミクスを理解するための 新しい道を開きます