系統特有の増強剤は,マクロファージと胚性幹細胞の自己再生遺伝子を活性化させる
Erinn L Soucie1, Ziming Weng2, Laufey Geirsdóttir3
1Centre d'Immunologie de Marseille-Luminy, Université Aix-Marseille, UM2, Campus de Luminy, Case 906, 13288 Marseille Cedex 09, France. INSERM, U1104, Marseille, France. CNRS, UMR 7280, Marseille, France. Centre de Recherche en Cancerologie de Marseille, INSERM (U1068), CNRS (U7258), Université Aix-Marseille (UM105), Marseille, France. sieweke@ciml.univ-mrs.fr erinn.soucie@inserm.fr arend@stanford.edu.
Science (New York, N.Y.)
|January 23, 2016
まとめ
成熟したマクロファージは,MafBとc-Mafの転写因子によって制御される自己再生能力を有する. これらの要因は 自己再生に不可欠な遺伝子ネットワークを制御し 胚性幹細胞にも存在します
科学分野:
- 免疫学
- 細胞生物学
- 遺伝学
背景:
- 分類されたマクロファージは組織と長期培養で自己再生を示します.
- マクロファージの自己更新を制御する正確な遺伝子調節メカニズムは,ほとんど不明のままです.
研究 の 目的:
- 分化されたマクロファージの自己再生を制御する遺伝子調節機構を解明する.
- マクロファージの自己再生に関与する重要な転写因子と遺伝子ネットワークを特定する.
主な方法:
- マウスモデルを in vivo 研究に使用した.
- マクロファージの集団を調査するために単細胞分析を用いた.
- 転写因子MafBとc-Mafの役割を調査した.
主要な成果:
- MafBとc-Mafは,自己再生を制御するマクロファージ特有の増強ネットワークの抑制剤として特定されました.
- このネットワークにアクセスするために,増殖するマクロファージが一時的にマフの転写因子をダウンレギュレーションすることを示した.
- マクロファージと胚性幹細胞の間で共有される 自己更新遺伝子のネットワークを明らかにし, 異なる増強剤のプラットフォームによって制御されます.
結論:
- MafBとc-Mafの転写因子はマクロファージの自己再生の重要な調節因子である.
- 保存された遺伝子ネットワークは 系統特有の増強剤によって調節された 異なる細胞タイプにおける自己再生を制御する.
- これらのメカニズムを理解することで 組織再生や幹細胞生物学への洞察が得られます
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