Cu ((2+) は,フォスファディチルエタノアミンと結合し,脂質膜の酸化を増加させる

Matthew F Poyton¹, Anne M Sendecki¹, Xiao Cong²

¹Department of Chemistry, Penn State University , University Park, Pennsylvania 16801, United States.

Journal of the American Chemical Society

|January 29, 2016

まとめ

銅イオン (Cu2+) は,フォスファディテレタノアミン (PE) 脂質に結合し,酸化を加速する. これは高濃度の銅と関連した神経変性疾患や自閉症における脂質酸化を説明するかもしれない.

科学分野:

生物化学
バイオ物理学
神経科学

背景:

リン酸エタノアミン (PE) は哺乳類の細胞膜に含まれる主要な脂質である.
銅 (Cu2+) 不調は神経変性疾患と自閉症に関連しています.

研究の目的:

Cu2+とPE脂質の結合相互作用を調査する.
PE脂質酸化に対するCu2+の効果を決定する.

主な方法:

異なるpHとPE濃度で測定された,見かけの均衡解離定数 (K ((DApp))
Cu2+と過酸化水素を用いて,PEを含む二重層における脂質二重結合の酸化をモニターする.

主要な成果:

Cu2+は,生理的なpHで約2μMのK ((DApp)) を持つPE脂質に二価結合する.
pH10.0では,PE濃度 (150 nM 70 mol% PE) により,PEへのCu2+結合親和性が増加する.
PEを含む二重層は,Cu2+と過酸化水素の存在において,純粋なフォスファディチルコリン (PC) 二重層よりも8.2倍速い酸化速度を示します.

結論:

PE脂質へのCu2+結合は,酸化に対する感受性を高めます.
PEが豊富な膜における脂質酸化の増加は,高銅レベルに関連した神経変性疾患および自閉症の病理に寄与する可能性があります.

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