Limb Mesenchymeからの空間的に制限された信号によって指定されたプロリオセプターの筋肉型アイデンティティ
Sebastian Poliak1, Amy L Norovich2, Masahito Yamagata3
1Department of Neuroscience, Biochemistry, and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Cell
|January 30, 2016
まとめ
身体の位置を感知するプロピオセプターは 肢体の信号から 筋肉型のアイデンティティを得ます これらの感覚ニューロンの遺伝子発現の違いが 発達中の特定の筋肉と 繋がっていることを示しています
科学分野:
- 神経科学
- 発達生物学
- 遺伝学
背景:
- 感受性ニューロンは 特定の筋肉の標的を精密に刺激し 異なる筋肉型のアイデンティティを示します
- プロピオセプターアイデンティティの分子基礎と発達の起源はほとんど不明である.
研究 の 目的:
- プロピオセプターのアイデンティティを定義する 分子差を特定する.
- これらのプロピオセプター差異の発達起源を調査する.
主な方法:
- プロピオセプターで異なった発現を持つ遺伝子を特定するためにスクリーンが行われました.
- カデリン13 (cdh13),セマフォリン5a (sema5a),軟骨酸タンパク質-1 (crtac1) の発現パターンを分析した.
- Limb mesenchymeは,プロプロイセプター遺伝子発現への影響を評価するために遺伝子操作されました.
主要な成果:
- 抗体筋肉を制御するプロピオセプターは 異なった遺伝子発現プロファイルを示した.
- cdh13,sema5a,およびcrtac1の発現は,特定の肢域 (背中部と近距離) を神経化するプロイオセプターサブセットに限定された.
- 末端メゼンキームの 背中部特性を変化させると 自身の受容体の遺伝子発現プロファイルが 変化します
結論:
- プロピオセプターは 筋肉の標的と相関する 分子差異を示します
- 肢体メゼンキームは,プロピオセプターのアイデンティティに影響を与える信号を提供します.
- これらの信号は 発達中の四肢の近距離軸と中背軸に沿ってパターン化されています
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