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受容体ヘテロメアは,植物における雌性アトラクタンの男性の知覚を媒介する
Tong Wang1,2, Liang Liang1,2, Yong Xue1,2
1State Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Nature
|February 11, 2016
まとめ
研究者らは花粉管のMDIS1-MIK受容体を特定し,アラビドプシスの雌性アトラクタントLURE1を検出しました. この発見により 植物繁殖に不可欠な 女性の信号を 男性が認識する 新しいメカニズムが明らかになりました
科学分野:
- 植物の繁殖
- 分子生物学
- セル・シグナル
背景:
- 花の植物の性繁殖には 花粉管が卵子へと移動する 必要があります
- 雌性ゲメトファイトが分泌するペプチド (LURE1のような) は花粉管を惹きつけるが,雄性受容体は未確認である.
- この相互作用を理解することは 植物育種と生殖分離の鍵です
研究 の 目的:
- アラビドプシス・タリアナの LURE1 を感知する男性受容体を特定する.
- 花粉管によるLURE1の知覚のメカニズムを解明する.
主な方法:
- 酵母2ハイブリッドアッセイと共同免疫プレシピテーションでタンパク質の相互作用を研究する.
- 野生型と変異したアラビドプシスのLURE1に対する花粉管の成長の分析.
- バイオケミカルアッセイで受容体キナーゼの活性を調べる
- 種間の変容実験だ
主要な成果:
- 細胞表面受容体ヘテロマーMDIS1-MIKは花粉管で特定されました.
- MDIS1,MIK1,MIK2は,プラズマ膜局所化された受容体のようなキナーゼとして特徴づけられました.
- LURE1は特にMDIS1,MIK1,MIK2の細胞外ドメインと結合する.
- MDIS1またはMIK遺伝子の変異は,LURE1に対する花粉管の感受性を低下させた.
- LURE1誘発受容体二酸化とMIK1キナーゼ活性化
- アラビドプシスMDIS1の発現は,カプセラ・ルーベラで部分的に生殖隔離を克服した.
結論:
- MDIS1-MIKヘテロメアは,花粉管の雌性アトラクタLURE1の受容体として作用する.
- この相互作用には,特定の結合,受容体二分化,キナーゼ活性化が含まれます.
- この発見は,開花植物における雄性ゲメットの誘導のための新しいメカニズムを明らかにした.
- この受容体系は,種間の生殖互換性において重要な役割を果たします.
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