ESCRT IIIは,細胞移動中の核膜の破裂を修復し,DNAの損傷と細胞死を制限する

M Raab¹, M Gentili², H de Belly³

¹Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France. Institut Pierre-Gilles de Gennes, PSL Research University, F-75005 Paris, France.

Science (New York, N.Y.)

|March 26, 2016

まとめ

移転する細胞の核封筒はインターフェーズ中に一時的に開き,漏れとDNA損傷を可能にします. 効率的な核膜修復とDNA修復は狭い空間での細胞生存に不可欠です

科学分野:

細胞生物学
分子生物学
遺伝学

背景:

核膜は真核細胞における重要な障壁であり,核と細胞質の間の輸送を制御する.
この障壁は通常,ミトーシス時にのみ溶解する.

研究の目的:

インターフェーズ中の移動する哺乳類の細胞の核封筒のダイナミックな振る舞いを調査する.
移動する細胞の核封筒の開くメカニズムと結果を理解する.

主な方法:

移動する哺乳類の細胞の生体画像
核封筒の整合性とタンパク質の密輸の分析
DNAの損傷と修復メカニズムの評価
核封筒修復におけるESCRT (輸送に必要な内分体分類複合体) の役割の調査.

主要な成果:

核の変形により,移動する細胞のインターフェーズ中に核封筒が頻繁に開く.
核タンパク質は漏れ出し細胞質タンパク質はこれらの開口から漏れ込みます
DNAの二重鎖の断裂は核封筒の開くイベントと関連しています.
ESCRT経路は核の封筒を迅速に修復するために不可欠です.

結論:

細胞移動中に核封筒が開くことは,ゲノムの完全性に影響を与える重要な出来事です.
閉じ込められた環境における細胞の生存は堅固な核膜とDNA修復システムに依存しています
移動する白血球における一時的な核封筒開口は,免疫反応に影響を与える可能性があります.

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