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遺伝子回路設計の自動化
Alec A K Nielsen1, Bryan S Der2, Jonghyeon Shin1
1Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
まとめ
DNAでコード化された 遺伝子回路の設計環境を 開発しました この自動化は,生物技術の応用のための複雑な生物回路の作成を簡素化し,意思決定と制御を改善します.
科学分野:
- 合成生物学
- コンピュータ生物学
- 分子工学
背景:
- 生きている細胞は DNAでコード化された回路を使って 計算を行うことができます
- これらの回路の構築は,手作業の組み立てと表現バランスにより,現在時間がかかります.
研究 の 目的:
- DNAでコードされた遺伝子回路の作成を 自動化する設計環境のCelloを紹介します
- 生物技術における遺伝子回路の設計プロセスを簡素化し,加速する.
主な方法:
- ユーザはVerilogのコードを書き セルロはDNAの配列に変換します
- アルゴリズムは回路設計,ゲート割り当て,接続,パフォーマンスシミュレーションを処理します.
- 遺伝子回路の設計はEscherichia coliでテストされました.
主要な成果:
- セルロはエシェリキア・コライの 60の遺伝子回路を 設計することに成功しました
- 設計された60回路のうち45回がすべての出力状態で正しく実行された.
- すべての設計された出力状態の92%がソフトウェアによって予測されたように機能した.
結論:
- セルロース設計の自動化は 遺伝子回路をバイオテクノロジーに組み込むことを簡素化します
- このアプローチは,生物学的システムにおける意思決定,制御,感知,空間的組織を必要とするアプリケーションを容易にする.
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