Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.7K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.7K
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

2.5K
2.5K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

4.3K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
4.3K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

6.3K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.3K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

2.3K
2.3K
Lineage Commitment01:21

Lineage Commitment

4.5K
Commitment is the  process whereby stem cells:
4.5K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Autonomous biomedical research with an artificial intelligence agent.

Science (New York, N.Y.)·2026
Same author

Unified Transcriptome and Mechanics Map of the Intact Mammalian Preimplantation Embryo In Situ.

bioRxiv : the preprint server for biology·2026
Same author

Disordered protein LAT encodes relative levels of signaling pathways in T cell activation.

Science (New York, N.Y.)·2026
Same author

Experimental and computational methods for allelic imbalance analysis from single-nucleus RNA-seq data.

Genome biology·2026
Same author

Learning multi-cellular representations of single-cell transcriptomics data enables characterization of patient-level disease states.

Cell systems·2026
Same author

Mutant ribosomal protein RPS15 drives B cell malignancy through oxidative stress and genomic instability.

Nature communications·2026

関連する実験動画

Updated: Mar 22, 2026

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

2.0K

コア・サーカディアン・クロック・ゲンはAMLにおける白血病幹細胞を調節する

Rishi V Puram1, Monika S Kowalczyk2, Carl G de Boer2

  • 1Department of Medicine, Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of Harvard University and MIT, Cambridge, MA 02142, USA.

Cell
|April 9, 2016
PubMed
まとめ

コア・サーカディアン・クロック遺伝子であるClockとBmal1は,急性骨髄性白血病 (AML) の細胞成長に不可欠です. これらの遺伝子を破壊すると,白血病の進行が止まり,白血病幹細胞 (LSCs) が枯渇します.

さらに関連する動画

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
09:01

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

14.9K
Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
09:09

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Published on: November 10, 2017

8.9K

関連する実験動画

Last Updated: Mar 22, 2026

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

2.0K
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
09:01

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

14.9K
Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
09:09

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Published on: November 10, 2017

8.9K

科学分野:

  • 血液学
  • 分子生物学
  • クロノバイオロジー

背景:

  • 白血病幹細胞 (LSCs) は急性骨髄性白血病 (AML) を誘発し,重要な治療標的である.
  • LSCの生存と増殖に不可欠な遺伝子を特定することは,治療性AML治療法の開発の鍵です.

研究 の 目的:

  • 急性骨髄性白血病 (AML) の白血病幹細胞 (LSC) 機能のための必須転写因子 (TFs) を特定する.
  • AMLの病原化とLSCの維持における昼夜時計の役割を調査する.

主な方法:

  • ネズミのAMLモデルで,集合したin vivoRNA干渉 (RNAi) スクリーンを使用した.
  • AML細胞の増殖,分化,およびLSCの枯渇における特定された遺伝子の機能を分析した.
  • 遺伝的ノックアウトモデルを用いて,白血病特有の循環経路への依存性を評価した.

主要な成果:

  • AML細胞の生体内および体内生殖に不可欠な,昼夜リズム転写因子であるClockとBmal1を特定した.
  • 細胞の生理経路の構成要素の障害は,白血病の増殖の障害,骨髄分泌の強化,およびLSCの枯渇を含む抗白血病効果を発揮することを示した.
  • 正常細胞と悪性細胞の両方に 機能的な生体時計があることが確認されましたが AMLはこの経路に特異的な依存を示しています

結論:

  • コア・サーカディアン・クロック・ゲンは 急性骨髄性白血病の生物学と伝播に 重要な役割を果たします
  • AMLにおけるサーカディアン経路は治療の脆弱性であり,治療開発の新たな道筋を提供する.