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Updated: Mar 22, 2026

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

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イノシトールポリフォスファートセンサドメインによるユーカリオット・フォスファート・ホメオスタシスの制御

Rebekka Wild1, Ruta Gerasimaite2, Ji-Yul Jung3

  • 1Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland.

Science (New York, N.Y.)
|April 16, 2016
PubMed
まとめ

細胞は,イノシトールポリフォスファート (InsPs) を結合するSPXドメインを使用して,リン酸 (P(i)) を感知する. この相互作用は,ユカリオットの間でリン酸の吸収,輸送,貯蔵を調節し,保存されたシグナルメカニズムを明らかにします.

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Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
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