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RNA Structure01:23

RNA Structure

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29.7K
RNA Structure01:23

RNA Structure

80.2K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
80.2K
RNA Structure01:19

RNA Structure

8.2K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
8.2K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

33.5K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
33.5K
Bacterial Transcription01:53

Bacterial Transcription

38.6K
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
38.6K
RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Updated: Mar 22, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

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3D RNAと進化的結合による機能的相互作用

Caleb Weinreb1, Adam J Riesselman2, John B Ingraham1

  • 1Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.

Cell
|April 19, 2016
PubMed
まとめ
この要約は機械生成です。

この研究は,RNAの構造と機能を予測するために進化配列分析を使用しています. この方法はRNAとRNAタンパク質の相互作用を正確にモデル化し,新しいRNA遺伝子の発見を加速します.

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Analyzing and Building Nucleic Acid Structures with 3DNA
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Analyzing and Building Nucleic Acid Structures with 3DNA

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

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Analyzing and Building Nucleic Acid Structures with 3DNA
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Analyzing and Building Nucleic Acid Structures with 3DNA

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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科学分野:

  • コンピューター生物学
  • 分子生物学
  • バイオ情報学

背景:

  • 非コーディングRNAは豊富ですが,その構造と機能はよく理解されていません.
  • 新しいRNA遺伝子配列の発見は 機能的役割や構造的特性に関する研究を上回ります
  • RNAの構造と機能を理解することは 生物学的プロセスを解読するのに不可欠です

研究 の 目的:

  • RNAの構造と機能を予測するための進化配列データを活用する.
  • RNAとRNAタンパク質複合体内の核酸-核酸および核酸-アミノ酸の相互作用を推論する.
  • 既知のRNA分子と未知のRNA分子の両方の正確な3D構造予測を可能にします.

主な方法:

  • 配列共変数の最大エントロピーグローバル確率モデルを使用した.
  • 進化のシーケンス記録を分析して 制限された相互作用を特定した.
  • RNAとRNA-タンパク質複合体の構造を予測するために,進化的結合分析を適用した.

主要な成果:

  • 既知のRNA構造と複合体に対する正確な全原子盲の3D構造予測を達成した.
  • 未知の構造を持つ160の非コーディングRNAファミリーとの接触が予測されています.
  • リボスイッチスイッチポイントとHIV核形成部位を含む重要な機能的相互作用が特定されました.

結論:

  • 進化的結合分析はRNAの3D構造を予測する強力なツールです
  • このアプローチは,RNA分子と複合体の重要な機能的相互作用を効果的に明らかにします.
  • 配列データを増加させることで RNAの発見を加速する進化的結合の力を高めます