細胞内標的の分子磁気共振イメージングのための膜浸透性Mn (III) コンプレックス
Ali Barandov1, Benjamin B Bartelle1, Beatriz A Gonzalez1
1Departments of †Biological Engineering, ‡Chemistry, §Brain and Cognitive Sciences, and ⊥Nuclear Science and Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
Journal of the American Chemical Society
|April 19, 2016
まとめ
研究者らは,磁気共鳴画像 (MRI) のための新しいマンガン (Mn(III)) ベースのコントラスト剤を開発した. これらの探査機は細胞内空間を効果的に標的とし 細胞内の分子画像を 強化します
科学分野:
- バイオメディカルイメージング
- 分子イメージング
- ナノテクノロジー
背景:
- 細胞内は重要ですがMRIで画像を撮るのは困難です
- 標準的なMRIコントラスト剤は 細胞膜の浸透性を阻害します
- 高解像度のMRIは,細胞内分析物質の検出に敏感な探査機を必要とします.
研究 の 目的:
- 新しいMRIコントラスト剤を開発し,細胞内での効果的投射とイメージングを行う.
- 細胞内標的の 分子MRIのプラットフォームを作る
- 細胞空間へのアクセスにおける現在のMRIコントラスト剤の限界を克服する.
主な方法:
- 1,2-フェニレンダイアミド (PDA) の骨格を持つ合成された平面テトラデンタートMn(III) ケラート.
- Mn (III) -PDA複合体の自発的な細胞局所化メカニズムを調査した.
- 細胞蓄積のための酵素分解性アセトメトキシエステル群を持つ探査機変種を開発した.
- 標的細胞の標識化のための基板選択エステラーゼ発現を利用した.
主要な成果:
- Mn(III) - PDA複合体は,ガドリニウム (Gd(III)) 剤に匹敵するT1緩解性を示した.
- 細胞溶液への自発的な局所化が示された.
- 細胞内で効果的に蓄積する.
- 細胞内検出が成功したことを示す,強固なT1加重型MRI強化が達成されました.
結論:
- Mn(III) -PDA複合体は,細胞内標的の分子MRIに有効な戦略を提供します.
- これらの薬剤は,細胞内イメージングのための反応剤を開発するための多用途のプラットフォームを提供します.
- この研究は,新しいMn (III) 探査機を使用して細胞内分析剤を検出するための先例を確立しています.
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