Term-seqは,細菌における抗生物質耐性の豊富なリボ調節を明らかにする
Daniel Dar1, Maya Shamir1, J R Mellin2
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
Science (New York, N.Y.)
|April 28, 2016
まとめ
研究者らは細菌の遺伝子発現を制御する 新種の抗生物質に反応する RNA 調節物質を発見した. この研究は,様々な代謝物質に反応するRNAスイッチを特定するための新しいツールを提供し,抗生物質耐性研究を支援しています.
科学分野:
- 細菌の遺伝子調節
- RNA生物学
- 抗菌剤耐性
背景:
- リボスイッチと減衰剤は,細菌の遺伝子発現を制御するシス調節RNA要素である.
- これらの要素は通常,メタボライト結合に反応して早めの転写終了を媒介する.
研究 の 目的:
- バクテリアのリボレギュレータを発見するための,偏らない,全ゲノム的な方法を開発する.
- メタボリート反応性レギュレータの in vivo 活性とスクリーニングを定量化するためのプラットフォームを作成します.
主な方法:
- ゲノム全体のリボレギュレータの実験的発見
- 高通量 in vivo 活動測定とスクリーニングプラットフォーム
- Listeria monocytogenesの特定の調節体の詳細なメカニズム研究
主要な成果:
- 病原体とヒトの微生物群における多数の抗生物質反応性リボレギュレータを特定した.
- Listeria monocytogenesの抗生物質が停止したリボソームを含む衰弱メカニズムを特徴づけた.
- 抗生物質耐性調節における条件付き終止の広範な役割が示されています.
結論:
- 抗生物質耐性を制御する上で重要な役割を果たします.
- 開発されたアプローチは,多様なリガンド特異性を有する新しいリボスイッチと減衰剤を発見するための貴重なツールとして機能します.
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